Integrated Electrochemical Oxidation and Biodegradation for Remediation of a Neonicotinoid Insecticide Pollutant.

A novel integrated electrochemical oxidation (EO) and bacterial degradation (BD) technique was employed for the remediation of the chloropyridinyl and chlorothiazolyl classes of neonicotinoid (NEO) insecticides in the environment. Imidacloprid (IM), clothianidin (CL), acetamiprid (AC), and thiamethoxam (TH) were chosen as the target NEOs. Pseudomonas oleovorans SA2, identified through 16S rRNA gene analysis, exhibited the potential for BD. In EO, for the selected NEOs, the total percentage of chemical oxygen demand (COD) was noted in a range of 58-69%, respectively. Subsequently, in the biodegradation of EO-treated NEOs (BEO) phase, a higher percentage (80%) of total organic carbon removal was achieved. The optimum concentration of NEOs was found to be 200 ppm (62%) for EO, while for BEO, the COD efficiency was increased up to 79%. Fourier-transform infrared spectroscopy confirms that the heterocyclic group and aromatic ring were degraded in the EO and further utilized by SA2. Gas chromatography-mass spectroscopy indicated up to 96% degradation of IM and other NEOs in BD (BEO) compared to that of EO (73%). New intermediate molecules such as silanediamine, 1,1-dimethyl-n,n'-diphenyl produced during the EO process served as carbon sources for bacterial growth and further mineralized. As a result, BEO enhanced the removal of NEOs with a higher efficiency of COD and a lower consumption of energy. The removal efficiency of the NEOs by the integrated approach was achieved in the order of AC > CL > IM > TH. This synergistic EO and BD approach holds promise for the efficient detoxification of NEOs from polluted environments.

Efficient biogenesis of calcium oxide nanoparticles using the extract of Eleusine coracana seeds and their application against multidrug-resistant ocular bacterial pathogens.

Visual impairment due to corneal keratitis-causing bacteria is becoming a matter of health concern. The bacterial colonization and their resistance to multiple drugs need imperative attention. To overcome the issue of alternative remedial therapeutic agents, particularly for topical application, a study was carried out to synthesize calcium oxide nanoparticles (CaO NPs) using the biomaterial Eleusine coracana seed aqueous extract. The biosynthesized calcium oxide nanoparticles (CaO NPs) are non-toxic or less-toxic chemical precursors. Moreover, CaO NPs are eco-friendly and are used for several industrial, biomedical, and environmental applications. Biosynthesized CaO NPs were characterized using ultraviolet-visible spectroscopy, Fourier transform-infrared spectroscopy, scanning electron microscopy, and dynamic light scattering study. The synthesized CaO NPs exhibit with good anti-inflammatory activities with dose dependant (50-250 µg/mL). Moreover, Eleusine coracana-mediated CaO NPs significantly inhibited the multiple drug-resistant Gram-positive Staphylococci epidermidis and Enterococcus faecalis and Gram-negative Escherichia coli and Klebsiella pneumoniae that were isolated from the corneal ulcer. This study provides a potential therapeutic option for multiple drug-resistant corneal pathogens that cause vision impairment.

Influence of Polar Solvents on Third-Order Nonlinear Optical Features of Methyl Red Dye.

This study emphasis the solvent effect on third-order nonlinear optical (NLO) features of methyl red (MR) dye dissolved in polar solvents including ethanol, methanol, acetone, 1-propanol, DMF and DMSO using low power diode laser. Z-scan technique operating at 405 nm wavelength, is used to estimate the third-order NLO features of MR dye in various solvents. The dye discloses self-defocusing nonlinear index of refraction (n2), which is determined to be the order of 10-7 cm2/W. The nonlinear coefficient of absorption (ß) of MR dye displays both negative and positive value owing to saturable absorption (SA) and reverse saturable absorption (RSA), respectively. The real and imaginary components of the third-order NLO susceptibility of MR dye in polar solvents are measured to be the order of 10-6 esu and 10-7 esu, respectively. The dye exhibits a large NLO susceptibility in DMSO, which is estimated to be 1.21 × 10-6 esu. The effect of solvent spectral features on MR dye is determined by applying a multi-parameter scale called Kamlet-Abboud-Taft. The experiment results indicate that MR dye is a promising NLO material that may find applications in photonics and optoelectronics.

Application of photoelectrochemical oxidation of wastewater used in the cooling tower water and its influence on microbial corrosion.

Background: Cooling towers are specialized heat exchanger devices in which air and water interact closely to cool the water's temperature. However, the cooling water contains organic nutrients that can cause microbial corrosion (MC) on the metal surfaces of the tower. This research explores the combined wastewater treatment approach using electrochemical-oxidation (EO), photo-oxidation (PO), and photoelectrochemical oxidation (PEO) to contain pollutants and prevent MC. Methods: The study employed electro-oxidation, a process involving direct current (DC) power supply, to degrade wastewater. MC studies were conducted using weight loss assessments, scanning electron microscopy (SEM), and x-ray diffraction (XRD). Results: After wastewater is subjected to electro-oxidation for 4 h, a notable decrease in pollutants was observed, with degradation efficiencies of 71, 75, and 96%, respectively. In the wastewater treated by PEO, microbial growth is restricted as the chemical oxygen demand decreases. Discussion: A metagenomics study revealed that bacteria present in the cooling tower water consists of 12% of Nitrospira genus and 22% of Fusobacterium genus. Conclusively, PEO serves as an effective method for treating wastewater, inhibiting microbial growth, degrading pollutants, and protecting metal from biocorrosion.

Nonlinear Optical Refraction and Absorption Features of Methyl Orange Dye in Polar Solvents.

Herein, we report the nonlinear optical (NLO) refraction and absorption features of azo dye namely, methyl orange (MO) dissolved in ethanol, methanol, acetone, 1-propanol, DMF and DMSO. The UV-Visible absorption study reveals that the maximum absorption spectrum of MO dye appeared towards longer wavelength by increasing the solvent polarizability is the result of red shift or bathochromic shift. The Z-scan method is utilized to measure the third-order NLO features of MO dye in different polar solvents. A continuous wave laser with 5-mW power and an excitation wavelength of 405 nm is employed in the Z-scan technique. The NLO features including nonlinear index of refraction (n2), nonlinear coefficient of absorption (ß) and third-order NLO susceptibility (χ3) are calculated to be the order of 10-7 cm2/W, 10-2 cm/W and 10-7 esu, respectively. The NLO index of refraction shows peak-valley transmittance is the result of self-defocusing and NLO absorption coefficient exhibits both positive and negative nonlinearity owing to saturable absorption (SA) and reverse saturable absorption (RSA). The effect of solvent polarizability and dipole moment on third-order NLO susceptibility of MO dye is discussed. Based on the experimental results, an azo dye MO appears to be a promising option for NLO applications in the future.

Development of an environmentally sustainable technique to minimize the sludge production in the textile effluent sector through an electrokinetic (EK) coupled with electrooxidation (EO) approach.

This study presents an environmentally sustainable method for minimizing sludge production in the textile effluent sector through the combined application of electrokinetic (EK) and electrooxidation (EO) processes. AAS and XRF analyses reveal that utilizing acidic electrolytes in the EK method successfully eliminates heavy metals (Cu, Mn, Zn, and Cr) from sludge, demonstrating superior efficiency compared to alkaline conditions. In addition, the total removal efficiency of COD contents was calculated following the order of EK-3 (60%), EK-1 (51%) and EK-2 (34%). Notably, EK-3, leveraging pH gradient fluctuations induced by anolyte in the catholyte reservoir, outperforms other EK systems in removing COD from sludge. The EK process is complemented by the EO process, leading to further degradation of dye and other organic components through the electrochemical generation of hypochlorite (940 ppm). At an alkaline pH of 10.0, the color and COD removal were effectively achieved at 98 and 70% in EO treatment, compared to other mediums. In addition, GC-MS identified N-derivative residues at the end of the EO. This study demonstrates an integrated approach that effectively eliminates heavy metals and COD from textile sludge, combining EK with EO techniques.

Solar-activated and hydrothermally synthesized effective rGO/Ag2S composites for the destruction of naphthol green B dye and antibacterial applications.

Graphene-based nanocomposites are developing as a new class of materials with several uses. The varied weight percentages of rGO on Ag2S catalysts were synthesized using a simple hydrothermal process and employed for the decomposition of anionic dye naphthol green B (NGB) under solar light. The reduced graphene oxide-based silver sulfide (rGO/Ag2S) nanoparticles were then examined using XRD, SEM, EDS, HR-TEM, XPS, UV-DRS, and PL analysis. Using solar light, the photocatalytic activity of the produced catalyst was examined for the degradation of naphthol green B (NGB) in an aqueous solution. At pH 9, rGO/Ag2S is discovered to be more effective than the other catalysts for the NGB dye mineralization. Analyses have been conducted on the influence of operational parameters on the photo-mineralization of NGB, including the initial pH, initial dye concentration, and catalyst dosage. The dye concentration increased; the efficiency of photocatalytic degradation tended to decrease. Chemical oxygen demand (COD) studies have verified the NGB dye mineralization. Active species trapping revealed that holes, hydroxyl radicals, and superoxide radicals all played major roles in the photocatalytic deterioration of NGB processes. Additionally, a potential mechanism of NGB dye degradation by rGO/Ag2S catalyst is presented. The synthesized compound was further evaluated for antibacterial activity, and the results indicated that rGO/Ag2S were potentially effective antibacterial agents.

Assessment of antimicrobial, antidiabetic, and anti-inflammatory properties of acetone extract of Aerva lanata (L.) by in-vitro approach and bioactive compounds characterization.

The antimicrobial, antidiabetic, and anti-inflammatory activities efficiency of Aerva lanata plant extracts (aqueous (Aqu-E), acetone (Ace-E), and ethanol (Eth-E)) were investigated in this study. Furthermore, the active molecules exist in the crude extract were characterized by UV-Visible spectrophotometer, Fourier transform infrared (FTIR), High-performance liquid chromatography (HPLC), and Gas Chromatography-Mass Spectrometry (GC-MS) analyses. The preliminary phytochemical study revealed that the Ace-E restrain more phytochemicals like alkaloids, saponins, anthraquinone, tannins, phenolics, flavonoids, glycosides, terpenoids, amino acid, steroids, protein, coumarin, as well as quinine than Aqu-E and Eth-E. Accordingly to this Ace-E showed considerable antimicrobial activity as the follows: for bacteria S. aureus > E. coli > K. pneumoniae > P. aeruginosa > B. subtilis and for fungi T. viride > A.flavus > C. albicans > A.niger at 30 mg ml concentration. Similarly, Ace-E showed considerable antidiabetic (α-amylase: 71.7 % and α-glucosidase: 70.1 %) and moderate anti-inflammatory (59 % and 49.8 %) activities. The spectral and chromatogram studies confirmed that the Ace-E have pharmaceutically valuable bioactive molecules such as (Nbutyl)-octadecane, propynoic acid, neophytadiene, and 5,14-di (N-butyl)-octadecane. These findings suggest that Ace-E from A. lanata can be used to purify additional bioactive substances and conduct individual compound-based biomedical application research.

Synthesis of iron oxide nanoparticles using orange fruit peel extract for efficient remediation of dye pollutant in wastewater.

The removal of color-causing compounds from wastewater is a significant challenge that industries encounter due to their toxic, carcinogenic, and harmful properties. Despite the extensive research and development of various techniques with the objective of effectively degrading color pollutants, the challenge still persists. This paper introduces a simple technique for producing iron oxide nanoparticles (Fe2O3 NPs) using orange fruit peel for sustainable dye degradation in aqueous environment. The observation of color change and the measurement of UV-visible absorbance at 240 nm provided a confirmation for the development of Fe2O3 NPs. Transmission electron microscopy examination demonstrated that the Fe2O3 NPs have an agglomerated distribution and forming spherical structures with size ranging from 25-80 nm. Energy-dispersive X-ray spectroscopy analysis supported the existence of Fe and O. Fourier transform infrared spectroscopy conducted to investigate the involvement of orange peel extract in the reduction, capping, and synthesis of Fe2O3 NPs from the precursor salt. Fe2O3 NPs showed a photocatalytic remediation of 97%, for methylene blue under visible light irradiation. Additionally, prepared NPs exhibited concentration depended biofilm inhibition action against E. coli and S. aureus. In conclusion, Fe2O3 NPs can efficiently purify water and suppress pathogens due to their strong degrading activity, reusability, and biofilm inhibition property.

Identification and characterization of major bioactive compounds from Andrographis paniculata (Burm. f.) extracts showed multi-biomedical applications.

The Andrographis paniculata recognized as most valuable medicinal plant in folk medicine. Hence, this research was designed to evaluate antibacterial potential of petroleum ether (PE) and methanol (ME) extracts of A. paniculata against skin infection causing bacterial pathogens such as Staphylococcus aureus, Streptococcus pyogenes, Klebsiella pneumoniae, Enterobacter aerogenes, Proteus vulgaris, and Propionibacterium acnes. Also assessed the antidiabetic (α-glucosidase and α-amylase inhibition assay), antioxidant, and photoprotective potential of PE and ME extract analyses. The major bioactive compounds were identified and characterized through UV, FTIR, 1H-NMR and 13C-NMR spectra analyses. The ME extract contain more number of phytochemicals (alkaloids, flavonoids, saponins, terpenoids, glycoside, protein, and phytosterol) than PE extract. The antibacterial activity result also revealed that the ME (as dose dependent) extract showed better activity at 250 mg mL-1 as in the following order: P. acnes (6-29 mm) > K. pneumoniae (3-28 mm) > S. aureus (3-27 mm) > P. vulgaris (3-26 mm) > S. pyogenes (2-25 mm) > E. aerogenes (1-23 mm). PE: E. aerogenes (3-20 mm) > P. vulgaris (2-19 mm) > P. acnes (3-18 mm) > K. pneumoniae (3-17 mm) > S. aureus (2-16 mm) > S. pyogenes (0-11 mm). The MIC value of ME extract was found as 100-150 mg mL-1 and it was better than PE extract. Similarly, the ME also possesses dose based α-glucosidase inhibition activity as up to 85% at 250 mg mL-1 concentration. The fluorescence spectra analysis method also stated that the ME extract possess photoprotective bioactive agent. The ME fractions (F01 and F02) obtained from TLC and column chromatogram were identified as 3-O-ß-d-glucosyl-14- deoxyandrographiside and 14-deoxyandrographolide respectively through UV, FTIR, 1H-NMR and 13C-NMR spectra analyses. Such compounds may be responsible for significant antibacterial activity against pathogenic bacteria causing skin infections, excellent antidiabetic activity, as well as photoprotective potential.

Biodegradation of pyrene by bacterial consortia: Impact of natural surfactants and iron oxide nanoparticles.

Polycyclic aromatic hydrocarbons (PAHs) are potentially hazardous compounds that could cause a severe impact on many ecosystems. They are very challenging to remove using conventional methods due to their hydrophobic nature. However, this issue can be resolved by utilizing surface-active molecules to increase their bioavailability. In this study, pyrene was chosen as the PAH compound to explore its degradability by the effect of individual bacterial strains (Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3) and mixed consortia (MC) along with natural surfactant derived from Sapindus mukorossi and iron oxide nanoparticles (NPs). Additionally, fatty acids esters, dipeptides, and sugar derivative groups were identified as potent bioactive components of natural surfactants. Various techniques, such as XRD, VSM, TEM, and FE-SEM with EDX, were utilized to characterize the pristine and Fenton-treated iron oxide NPs. The analytical results confirmed that the Fe3O4 crystal phase and spherical-shaped NPs exhibited excellent magnetic properties. The impact of natural surfactants and iron oxide NPs has significantly contributed to the biodegradation process, resulting in a prominent decrease in chemical oxygen demand (COD) levels. Gas chromatography-mass spectrometry (GC-MS) analysis showed that biodegradation systems produced primary hydrocarbon intermediates, which underwent oxidative degradation through Fenton treatment. Interestingly, synthesized iron oxide NPs effectively produced hydroxyl radical (•OH) during the Fenton reaction, which was confirmed by electron paramagnetic resonance (EPR) spectra, and the pristine iron oxide NPs underwent a material transformation observed. The study demonstrated an integrated approach for biodegradation and the Fenton reaction process to enhance the pyrene degradation efficiency (90%) compared to other systems. Using natural surfactants and iron oxide NPs in aquatic environments serves as a crucial platform at the interface of microorganisms and contaminated oil products. This interaction offers a promising solution for PAHs bioremediation.

Integrated approach of nano assisted biodegradation of anthracene by Pseudomonas aeruginosa and iron oxide nanoparticles.

Poly aromatic hydrocarbons (PAHs) are considered as hazardous compounds which causes serious threat to the environment dua to their more carcinogenic and mutagenic impacts. In this study, Pseudomonas aeruginosa PP4 strain and synthesized iron nanoparticles were used to evaluate the biodegradation efficiency (BE %) of residual anthracene. The BE (%) of mixed degradation system (Anthracene + PP4+ FeNPs) was obtained about 67 %. The FTIR spectra result revealed the presence of functional groups (C-H, -CH3, CC, =C-H) in the residual anthracene. The FESEM and TEM techniques were used to determine the surface analysis of the synthesized FeNPs and the average size was observed by TEM around 5-50 nm. The crystalline nature of the synthesized iron nanoparticles was confirmed by the observed different respective peaks of XRD pattern. The various functional constituents (OH, C-H, amide I, CH3) were identified in the synthesized iron nanoparticles by FTIR spectrum. In conclusion, this integrated nano-bioremediation approach could be an promising and effective way for many environmental fields like cleanup of hydrocarbon rich environment.

Enhancement of Thermal and Gas Barrier Properties of Graphene-Based Nanocomposite Films.

Poly(vinyl alcohol) (PVA), a naturally occurring and rapidly decomposing polymer, has gained significant attention in recent studies for its potential use in pollution preventive materials. Its cost-effectiveness and ease of availability as well as simple processing make it a suitable material for various applications. However, the only concern about PVA's applicability to various applications is its hydrophilic nature. To address this limitation, PVA-based nanocomposites can be created by incorporating inorganic fillers such as graphene (G). Graphene is a two-dimensional carbon crystal with a single atom-layer structure and has become a popular choice as a nanomaterial due to its outstanding properties. In this study, we present a simple and environmentally friendly solution processing technique to fabricate PVA and graphene-based nanocomposite films. The resulting composite films showed noticeable improvement in barrier properties against moisture, oxygen, heat, and mechanical failures. The improvement of the characteristic properties is attributed to the uniform dispersion of graphene in the PVA matrix as shown in the SEM image. The addition of graphene leads to a decrease in water vapor transmission rate (WVTR) by 79% and around 90% for the oxygen transmission rate (OTR) as compared to pristine PVA films. Notably, incorporating just 0.5 vol % of graphene results in an OTR value of as low as 0.7 cm m-2 day-1 bar-1, making it highly suitable packaging applications. The films also exhibit remarkable flexibility and retained almost the same WVTR values even after going through tough bending cycles of more than 2000 at a bending radius of 2.5 cm. Overall, PVA/G nanocomposite films offer promising potential for PVA/G composite films for various attractive pollution prevention (such as corrosion resistant coatings) and packaging applications.

In vitro analysis of iron oxide (Fe3O4) nanoparticle mediated degradation of polycyclic aromatic hydrocarbons (PAHs) and their antimicrobial activity.

To degrade anthracene, magnetite nanoparticles were produced using a simple co-precipitation process. The fabricated nanoparticles have been analyzed for structural and optical properties. XRD examination revealed that the produced Fe3O4 nanoparticles were cubic phase, having a mean crystallite dimension of 18.84 nm. DLS determined the hydrodynamic diameter of Fe3O4 nanoparticles to be 182 nm. UV-Vis research revealed that Fe3O4 nanoparticles absorb at 390 nm. A peak at 895 cm-1 in the FT-IR study indicated the metal-oxygen connection. The synthesized Fe3O4 nanoparticles demonstrated an effective photocatalytic performance towards anthracene degradation and was found to be 86.55%. Furthermore, Fe3O4 nanoparticles showed the highest antimicrobial activity against Bacillus subtilis was 19.43 mm. The present study is the first and foremost study determining the dual role of Fe3O4 nanoparticles towards bioremediation and biomedical applications.

Impact of biosurfactant produced by Bacillus spp. on biodegradation efficiency of crude oil and anthracene.

Biosurfactants are surface active molecules generated by various microorganisms, including bacteria, actinobacteria, algae, and fungi. In this study, bacterial strains are isolated from soil contaminated with used motor oil and examined for potential biosurfactant production. A minimum salt medium (MSM), with crude oil as the only carbon source, is used to isolate potential biosurfactant-producing bacterial strains. About 23 strains are isolated, and all are subjected to the primary screening methods for biosurfactant production. Based on the emulsification index, oil displacement, and drop collapse screening methods, two isolates with potential biosurfactant-producing ability are selected for further studies. The synthesis of biosurfactants, crude oil and anthracene biodegradation is carried out with strains DTS1 and DTS2. Both strains show significant outcomes in crude oil degradation. In addition, both strains can utilize anthracene as the sole carbon source. During the degradation course, changes in the growth conditions are continuously monitored by measuring turbidity and pH. In this degradation study, the biosurfactant production aptitude of the isolated strains plays an essential role in increasing the bioavailability of hydrophobic hydrocarbons. These strains are identified down to the molecular level by employing 16S rRNA gene sequencing, and the acquired sequences are submitted to get the accession numbers. These prospective strains can be utilized to remediate hydrocarbon-contaminated environments.

G-C3N4-Ag composite mediated photocatalytic degradation of phenanthrene - A remedy for environmental pollution.

In recent years, g-C3N4-Ag nanocomposite synthesis has gained considerable attention for its potential to treat polycyclic aromatic hydrocarbons (PAHs) and to act against bacteria and fungi. In this study, we present a novel approach to the synthesis of g-C3N4-Ag nanocomposite and evaluate its efficiency in both PAH removal and antimicrobial activity. The synthesis process involved the preparation of g-C3N4 by thermal polycondensation of melamine. The factors that affect the adsorption process of PAHs, like time, pH, irradiation type, and adsorbent dosage, were also evaluated. Isotherm models like Langmuir and Freundlich determined the adsorption capability of g-C3N4-Ag. In simulated models, phenanthrene was degraded to a maximum of 85% at lower concentrations of catalyst. The adsorption profile of phenanthrene obeys the pseudo-second-order and Freundlich isotherms pattern. The g-C3N4-Ag nanocomposite also exhibited antimicrobial activity against bacteria (Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumoniae) and fungi (Candida albicans). The present study is the first report stating the dual application of g-C3N4-Ag nanocomposite in reducing the concentration of PAH and killing bacterial and fungal pathogens. The higher adsorption capability proclaimed by g-C3N4-Ag nanocomposite shows the fabricated nanomaterial with great potential to remediate organic pollutants from the ecosystem.

Fabrication and characterization of manganese dioxide (MnO2) nanoparticles and its degradation potential of benzene and pyrene.

MnO2 nanoparticles have a wide range of applications, including catalytic abilities due to their oxygen reduction potential. Industrial processes and the burning of organic materials released PAHs into the biosphere which have adverse effects on living organisms when continually exposed. In this study, MnO2 nanoparticles were synthesized chemically using sodium thiosulphate as reducing agent. MnO2 nanoparticles were characterized using UV-visible adsorption spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). A X-Ray Diffraction Spectrophotometer (XRD), a Scanning Electron Microscopy - Energy Dispersive X-Ray Analyzer (SEM-EDAX), and Dynamic Light Scattering (DLS) were used to identify the crystalline nature and particle size of the fabricated MnO2 nanoparticles. Batch adsorption studies were conducted to identify the optimal conditions for better benzene and pyrene adsorption from aqueous solution using MnO2 nanoparticles. They are also effective in degrading benzene and pyrene by batch adsorption as determined by their adsorption isotherms and kinetics.

Enhancement of cell migration and wound healing by nano-herb ointment formulated with biosurfactant, silver nanoparticles and Tridax procumbens.

Introduction: Ointments are generally used as a therapeutic agent for topical medication or transdermal drug delivery, such as wound healing and skin lesions. Methods: In this study, Tridax procumbens plant extract (0.7 g/mL) was used to prepare herbal-infused oil as the oil phase and gelatin-stabilized silver nanoparticle (G-AgNPs) (0.3 g/mL) as the aqueous phase. To blend the oil and aqueous phases, rhamnolipid biosurfactant with a critical micelle concentration of 55 mg/L from strain Pseudomonas aeruginosa PP4 has been used for herb ointment preparation. The average size of the synthesized G-AgNPs was observed between 10-30 nm and confirmed as spherical-shaped particles by TEM analysis. Subsequently, GC-MS and FTIR characterization are used to confirm herb ointment's chemical and functional characteristics. Results: Based on the antibacterial studies, the highest microbial growth inhibition was observed for herb ointment, about 19.5 mm for the pathogen Staphylococcus aureus at the concentration of 100 µg/mL, whereas 15.5 mm was obtained for Escherichia coli, respectively. In addition, the minimum inhibitory concentration (MIC) assay showed negligible bacterial growth at 100 µg/mL for S. aureus and E. coli, respectively. Moreover, the cell viability assay for herb ointment exhibited low cytotoxic activity at higher concentrations (100 µg/mL) in Vero cell lines. In this study, wound scratch assay showed a significant cell migration rate (90 ± 2%) in 3 days of incubation than the control (62 ± 2%). Discussion: As a result, the biosurfactant-based nano-topical herb ointment revealed a low cytotoxic and higher cell migration capacity. Altogether, these findings highlighted the utility of this herb ointment in therapeutic applications such as wound healing.